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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Stem Cells. 2015 Oct 10;33(12):3643–3654. doi: 10.1002/stem.2214

Figure 1. DNMT3B7 homozygous E14.5 embryos have fewer numbers of HSPCs.

Figure 1

(A) Lineage (FITC)-, Sca-1 (PE)+, c-Kit (APC)+ cells were stained in fetal livers isolated from E14.5 embryos that were Wild-type (WT: white), hemizygous DNMT3B7 transgenic (Hemiz: light grey) and homozygous DNMT3B7 transgenic (Homoz: dark grey). Average percentages from n ≥ 5 per group ± SEM are plotted. Two-tailed Student’s t-test was used to determine statistical significance. (B) Representative plots analyzed using FlowJo to quantify the relative levels of the analyzed populations. (C) Lineage (FITC)-, CD48 (PE)+, CD150 (APC)+ cells were stained in fetal livers isolated from E14.5 embryos that were Wild-type (WT: white), hemizygous DNMT3B7 transgenic (Hemiz.: light grey) and homozygous DNMT3B7 transgenic (Homoz.: dark grey). Average percentages from n ≥ 4 per group ± SEM are plotted. Two-tailed Student’s t-test was used to determine statistical significance. (D) Representative plots analyzed using FlowJo to quantify the relative levels of the analyzed populations.