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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Stem Cells. 2015 Oct 10;33(12):3643–3654. doi: 10.1002/stem.2214

Figure 3. DNMT3B7 transgenic HSPCs reconstitute normal blood counts in male, but not in female recipients.

Figure 3

(A–D) Female and (E–H) Male recipients of wild-type (WT: white), hemizygous DNMT3B7 transgenic (Hemiz: light grey) and homozygous DNMT3B7 transgenic (Homoz: dark grey) cells at two weeks post-transplantation. Ten million cells isolated from fetal livers of E14.5 embryos of the three genotypes were transplanted into hormonally intact (solid bars) or hormonally compromised (hatched bars) females (A–D) or males (E–H) that had been lethally irradiated at 960 rads. (A), (E) Average WBC number (x1000/μL); (B), (F) lymphocyte number (x1000/μL); (C), (G) Hb concentration (g/dL); and (D), (H) platelet number (x1000/μL) from n ≥ 4 mice per genotype per gender are plotted ± SEM. Two-tailed Student’s t-test was used to determine statistical significance.