Skip to main content
. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Neurobiol Dis. 2015 Nov 12;86:29–40. doi: 10.1016/j.nbd.2015.11.006

Figure 3. Evaluation of the preventive effect of MTZ on Aβ-mediated mitochondrial CytC release by Western blot and ELISA.

Figure 3

Neuronal SH-SY5Y and glioma cells were challenged with Aβ42 (10 μM; 24h) in the presence or absence of MTZ (100 and 300 μM) and subsequently subjected to subcellular fractionation. CytC was evaluated in mitochondrial and cytosolic extracts by WB and ELISA. (a-c) Western blot analysis of mitochondrial (a) and cytosolic (c) extracts. After electrophoretic separation of the respective subcellular fractions and electrotransfer to PVDF, membranes were probed for CytC and β-actin as loading control. Band intensities after normalization to the respective loading controls are illustrated in the bar-graphs below the corresponding WB. Each WB is representative of duplicate independent experiments. (b-d) CytC quantitated by ELISA in the mitochondrial (b) and cytoplasmic (d) extracts. Bars represent mean ± SD of triplicate experiments. * indicates p<0.05, ** p<0.01, and *** indicates p<0.001