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. 2015 Aug 14;33(12):3504–3518. doi: 10.1002/stem.2122

Figure 5.

Figure 5

Enrichment and expression analysis of CRXp‐GFP+ cells. (A): Dot plots indicate flow‐sorted GFP+ cells. Human embryonic stem cells with CRXp‐GFP (not shown) and aggregates without CRXp‐GFP at the same culture stage with sample are used as a negative control. (B): Bar graph shows CRX expression in GFP‐positive and negative fractions. Data are shown as mean FPKM ±SD from RNA‐seq data, n = 3 independent experiments for GFP+ cells, two independent experiments for GFP− cells. (C): Clustering of biological replicates. Heat map of RNA‐seq data represents similarity of sorted samples from blue (highest) to light purple (smallest). (D): Heat map represents expression of cone and rod‐associated genes in flow‐sorted GFP+ cells. Refer also Supporting Information Figure S3A, S3B for nonphotoreceptor lineage‐associated genes. (E): Volcano plots indicate fold change in expression. Top 10 differentially expressed genes are shown from comparisons between day 37 versus day 47, day 37 versus day 67, and day 37 versus day 90. For transcription factor genes, refer also Supporting Information Figure S3C. Abbreviations: GFP, green fluorescent protein; NR, neural retina.