Overview of systems vaccinology methods. Systems vaccinology aims to collect and integrate data from all components of the immune response. A combination of standardized immune serologic assays with the high-throughput transcriptomic, proteomic, and metabolomic measurements will provide the opportunity to predict immunologic protection from vaccines. [153]. Reprinted from Seminars in Immunology, 25, Li, S. et al., Systems biological approaches to measure and understand vaccine immunity in humans, 209–218, 2013, with permission from Elsevier.