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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Mol Oral Microbiol. 2015 Sep 10;31(3):243–258. doi: 10.1111/omi.12116

Figure 1. IL-6 production from Human peripheral blood monocytes (HPBMs) after exposure to periodontal bacteria.

Figure 1

HPBM were stimulated with the indicated ratios of bacteria to HPBM (MOI: 10:1, 1:1 and 0.1:1). After 12h stimulation, the supernatants were collected and pro-inflammatory IL-6 release was analyzed by ELISA. As positive controls, cells were incubated with 50 ng/ml E. coli O55B5 LPS, 50 ng/ml NOD1 ligand iE-DAP and 50 ng/ml NOD2 ligand MDP (with 10 times gradient dilution). Cells were stimulated with culture medium alone as a negative control. Results shown as mean ± SD of triplicate samples are representative of three independent experiments. Asterisks indicate statistically significant to culture medium negative control (P < 0.05) as determined by One-way ANOVA with Bonferroni post-test analysis.