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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Hepatology. 2015 Aug 21;63(2):512–523. doi: 10.1002/hep.27973

Figure 5. Despite increase in hepatic BA synthesis rate, SC-435 treatment is associated with reduced biliary BA concentration.

Figure 5

Concentrations for the biological precursor 7α-hydroxy-4-cholesten-3-one (C4), which correlates with BA synthesis, were determined by tandem mass spectrometry in plasma samples obtained on DOT 14 (A). Bile was aspirated from the gallbladders on DOT14 and subjected to MS-based quantification of PC, cholesterol, and BA concentrations. The biliary molar ratios of PC/BA (linkage coefficient) were calculated based on these measurements, and mean values of the bile constituents were plotted in a ternary phase diagram. Control mdr2−/− mice plot in region A, which is a 2 phase zone of the Wang and Carey diagram, characterized by very low PC/BA ratio, cholesterol supersaturation and formation of cholesterol crystals. SC-435 treated mdr2−/− and both groups of wild type mice plot in the micellar 1 phase zone of the diagram (B). Statistical analysis: unpaired t test was applied to compare the data between the two groups (SC-435 vs control) in mdr2−/− and mdr2+/+ mice.