Table 1. siRNAs used in this study.
| siRNA | Sequence a | Position (nt) b |
|---|---|---|
| siIE1 | 5′-GGC CUU GUG GUA CUG CCU GUU-3′ | 277–295 |
| 3′-UU CCG GAA CAC CAU GAC GGA C-5′ | ||
| siIE2 | 5′-CCU UGU GGU ACU GCC UGA UUU-3′ | 279–297 |
| 3′-UU GGA ACA CCA UGA CGG ACU A-5′ | ||
| siIE3 | 5′-UUG UGG UAC UGC CUG AUA GUU-3′ | 281–299 |
| 3′-UU AAC ACC AUG ACG GAC UAU C-5′ | ||
| siIE4 | 5′-GUG GUA CUG CCU GAU AGG GUU-3′ | 283–301 |
| 3′-UU CAC CAU GAC GGA CUA UCC C-5′ | ||
| siIE5 | 5′-GGU ACU GCC UGA UAG GGU GUU-3′ | 285–303 |
| 3′-UU CCA UGA CGG ACU AUC CCA C-5′ | ||
| siIE6 | 5′-UAC UGC CUG AUA GGG UGC UUU-3′ | 287–305 |
| 3′-UU AUG ACG GAC UAU CCC ACG A-5′ | ||
| siIE7 | 5′-CUG CCU GAU AGG GUG CUU GUU-3′ | 289–307 |
| 3′-UU GAC GGA CUA UCC CAC GAA C-5′ | ||
| siIE8 | 5′-GCC UGA UAG GGU GCU UGC GUU-3′ | 291–309 |
| 3′-UU CGG ACU AUC CCA CGA ACG C-5′ | ||
| siIE9 | 5′-CUG AUA GGG UGC UUG CGA GUU-3′ | 293–311 |
| 3′-UU GAC UAU CCC ACG AAC GCU C-5′ | ||
| siIE10 | 5′-GAU AGG GUG CUU GCG AGU GUU-3′ | 295–313 |
| 3′-UU CUA UCC CAC GAA CGC UCA C-5′ | ||
| siIE11 | 5′-UAG GGU GCU UGC GAG UGC CUU-3′ | 297–315 |
| 3′-UU AUC CCA CGA ACG CUC ACG G-5′ | ||
| siIE12 | 5′-GGG UGC UUG CGA GUG CCC CUU-3′ | 299–317 |
| 3′-UU CCC ACG AAC GCU CAC GGG G-5′ | ||
| siIE13 | 5′-GUG CUU GCG AGU GCC CCG GUU-3′ | 301–319 |
| 3′-UU CAC GAA CGC UCA CGG GGC C-5′ | ||
| siIE14 | 5′-GCU UGC GAG UGC CCC GGG AUU-3′ | 303–321 |
| 3′-UU CGA ACG CUC ACG GGG CCC U-5′ | ||
| siIE15 | 5′-UUG CGA GUG CCC CGG GAG GUU-3′ | 305–323 |
| 3′-UU AAC GCU CAC GGG GCC CUC C-5′ | ||
| siIE16 | 5′-GCG AGU GCC CCG GGA GGU CUU-3′ | 307–325 |
| 3′-UU CGC UCA CGG GGC CCU CCA G-5′ | ||
| siIE17 | 5′-GAG UGC CCC GGG AGG UCU CUU-3′ | 309–327 |
| 3′-UU CUC ACG GGG CCC UCC AGA G-5′ | ||
| siIE18 | 5′-GUG CCC CGG GAG GUC UCG UUU-3′ | 311–329 |
| 3′-UU CAC GGG GCC CUC CAG AGC A-5′ | ||
| siIE19 | 5′-GCC CCG GGA GGU CUC GUA GUU-3′ | 313–331 |
| 3′-UU CGG GGC CCU CCA GAG CAU C-5′ | ||
| siIE21 | 5′-CGG GAG GUC UCG UAG ACC GUU-3′ | 317–335 |
| 3′-UU GCC CUC CAG AGC AUC UGG C-5′ | ||
| siIE21.5 | 5′-GGG AGG UCU CGU AGA CCG UUU-3′ | 318–336 |
| 3′-UU CCC UCC AGA GCA UCU GGC A-5′ | ||
| siIE22 | 5′-GGA GGU CUC GUA GAC CGU GUU-3′ | 319–337 |
| 3′-UU CCU CCA GAG CAU CUG GCA C-5′ | ||
| siIE22.5 | 5′-GAG GUC UCG UAG ACC GUG CUU-3′ | 320–338 |
| 3′-UU CUC CAG AGC AUC UGG CAC G-5′ | ||
| siIE23 | 5′-AGG UCU CGU AGA CCG UGC AUU-3′ | 321–339 |
| 3′-UU UCC AGA GCA UCU GGC ACG U-5′ | ||
| siRNA | Sequence | Position (nt) |
| siIE24 | 5′-GUC UCG UAG ACC GUG CAC CUU-3′ | 323–341 |
| 3′-UU CAG AGC AUC UGG CAC GUG G-5′ | ||
| siIE25 | 5′-CUC GUA GAC CGU GCA CCA UUU-3′ | 325–343 |
| 3′-UU GAG CAU CUG GCA CGU GGU A-5′ | ||
| si313 | 5′-CCC GGG AGG UCU CGU AGA CTT-3′ | 315–333 |
| 3′-TT GGG CCC UCC AGA GCA UCU G-5′ | ||
| siE | 5′-GUC UCG UAG ACC GUG CAU CA UU-3′ | 323–342 |
| 3′-UU CAG AGC AUC UGG CAC GUA GU-5′ | ||
| siIE318_27 | 5′-GGG AGG UCU CGU AGA CCG UGC ACC A UU-3′ | 318–342 |
| 3′-UU CCC UCC AGA GCA UCU GGC ACG UGG U-5′ | ||
| siIE320_25 | 5′-GAG GUC UCG UAG ACC GUG CAC CA UU-3′ | 320–342 |
| 3′-UU CUC CAG AGC AUC UGG CAC GUG GU-5′ | ||
| siIE322_23 | 5′-GGU CUC GUA GAC CGU GCA CCA UU-3′ | 322–342 |
| 3′-UU CCA GAG CAU CUG GCA CGU GGU-5′ | ||
| siIE316 | 5′-CCG GGA GGU CUC GUA GAC CdTdT-3′ | 316–334 |
| 3′-dTdT GGC CCU CCA GAG CAU CUG G-5′ | ||
| siIE322 | 5′-GGU CUC GUA GAC CGU GCA CdTdT-3′ | 322–340 |
| 3′-dTdT CCA GAG CAU CUG GCA CGU G-5′ | ||
| siIE319 | 5′-GGA GGU CUC GUA GAC CGU GCA-3′ | 319–339 |
| 3′-GC CCU CCA GAG CAU CUG GCA C-5′ |
a Underlined and boldface letters indicate 3' overhang sequence added to the 3'-ends of siRNAs.
b siRNA target positions are numbered according to the HCV replicon Con1 sequence (genotype 1b; GenBank accession number AJ238799).