Fig 3. Disruption of YJR116w makes cells hypersensitive to copper.

A. 10-fold serial dilutions of yjr116w∆, ypr114w∆ and yy∆∆ mutants were plated on SC containing 2% glucose and indicated concentrations of CuSO₄. B. WT, yjr116w∆ (116∆), or ypr114w∆ (114∆) cells harboring either empty pYES2NT vector (ev) or the same expressing YJR116w or YPR114w (114 or 116, respectively), were plated on SC with 2% galactose (Gal). C. Superoxide anion (O₂^-) levels of three independent clones of the same strains grown for 15 h in presence or absence of 2 mM CuSO₄ were assessed by staining cells with DHE. * yjr116w∆ cells showed a significantly higher percentage of DHE positive cells than WT (P = 0.0039). D. As A, but plates were incubated anaerobically and contained 5 mg ml^-1 Tween 80 and 20 μg ml^-1 ergosterol. The freckles are precipitates caused by these additions to the medium. In each plate section, in the upper and lower rows are WT and yjr116w∆ cells, respectively. E. Copper sensitivity of indicated mutants was tested as in A. F. First and 3^rd columns as in A, the others supplemented additionally with 20 mM NAC. The 3^rd and 4^th columns show the growth behavior of ρ^- strains lacking the respiratory chain. G, growth on copper and higher concentrations of NAC.