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. 2016 Jan 11;11(1):e0145831. doi: 10.1371/journal.pone.0145831

Fig 5. Effect of myriocin treatment on LCB levels.

Fig 5

A. Cells growing exponentially in YPD were further incubated without (upper plot) or with 20 μg ml-1 myriocin (lower plot) for 4 hrs and LCB levels in the lipid extracts were determined by ESI-MS. A similar pretreatment with 2 μg ml-1 of myriocin repressed LCB levels of ypr114w∆ and WT to the same degree, albeit less strongly (not shown). B. Plates containing myriocin with or without 20 mM NAC were incubated for 2 days (top sections W303 background, bottom sections BY4742 background). C. Strains in the W303 background were grown in YPD with or without myriocin (1.2 μg ml-1) for 24 h and superoxide anions were detected by staining with DHE. After 24 h all strains had grown to the same density.