Fig 6. Yjr116w∆ and ypr114w∆ make lower amounts of superoxide anions in stationary phase than WT and have an increased CLS.

A. Cells were stained with DHE for measurement of superoxide anion levels. Fluorescence and transillumination pictures were merged to illustrate the difference between positive and negative cells. B. Percentages of DHE positive cells amongst 200 cells were determined in three independent experiments and the difference between mutants and WT in the same condition was evaluated by student’s t-test with P< 0.05 = *, P< 0.005 = **. C. The chronological life spans were determined in diploid deletion strains by measuring the colony-forming units after different periods in water (see Materials and Methods). Data represent three biological replicates using different clones and the differences between mutants and WT were evaluated as above.