Table 1. Apis mellifera mRNA and small RNA composition in each sequenced sample.

Mature oocytes and 0–2 h, 0–6 h and 18–24 h haploid and diploid embryos were submitted to RNA-seq, and the number of reads generated (Raw data), mapped to the A. mellifera transcript database as described in the Material and Methods section.

			Number of reads
			Total (Raw data)	mapped	microRNA	Annoted genes (A. mellifera)	Noncode	rRNA	tRNA	Pathogens
mRNA libraries	Mature Oocyte		82208246	47939930	291	47780988	120439	7385	30	30797
	Diploid Embryos	0-2h	22902101	15502664	84	15492088	7622	334	2	2534
		0-6h	40486094	26661385	177	26619330	35564	598	6	5710
		0-24h	33295431	20751326	431	20631201	101424	1219	9	17042
	Haploid Embryos	0-2h	17417565	11526057	44	11515606	7407	439	3	2558
		0-6h	37086884	23655878	188	23623252	28863	374	8	3193
		0-24h	163202336	87698424	1812	87258581	308725	39799	65	89442
Small RNA Libraries	Mature Oocyte		32955026	898309	6256	1995689	928113	14165	1199	251353
	Diploid Embryos	0-2h	17060175	1249412	6879	176645	97534	442	87	5689
		0-6h	37487408	287276	6386	230815	65112	323	189	11023
		0-24h	40213259	333157	25588	235739	54577	314	394	16545
	Haploid Embryos	0-2h	9899631	10921	171	7245	3273	14	0	218
		0-6h	38030892	118550	1611	88706	23682	120	122	4309
		0-24h	32219748	733618	27115	500389	191466	798	115	13735