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. 2016 Jan 11;11(1):e0146640. doi: 10.1371/journal.pone.0146640

Fig 2. p300 mediated transactivation of the hiNOS or heterologous promoter.

Fig 2

(A) The –7.2 kb wild-type (WT) human iNOS promoter construct (Pr8) or deleted -5 to -6 kb enhancer region (Pr8-Del), were co-transfected into A549 cells with p300 expression vector. Basal and stimulated luciferase activities were determined 6 hours after cytokine mix (CM) stimulation. Relative luciferase activities (RLA) values are the means ± sd of at least three separate experiments performed in triplicate. *Indicates P < 0.05 vs. basal, # indicates P < 0.05 vs. control (B) The minimal TK promoter construct with ligated hiNOS enhancer was co-transfected into A549 cells with p300 expression vector. Co-transfection with empty vector served as control. Basal and stimulated luciferase activity was determined 6 hr after cytokine stimulation. Values shown are the means ± sd of at least three separate experiments performed in triplicate. *Indicates P < 0.05 vs. basal, # indicates P < 0.05 vs. control.