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. 2016 Jan 11;11(1):e0146554. doi: 10.1371/journal.pone.0146554

Fig 2. The involvement of pro-apoptotic intermediates in M. fortuitum infection.

Fig 2

HKM were pre-treated separately with indicated inhibitors or transfected with specific siRNAs prior to M. fortuitum infection and at 24 h p.i. apoptosis measured by (A) Hoechst 33342 staining (B) AV/PI staining and (C) relative caspase-3 activity. STS treated HKM were analysed 6 h post incubation. Vertical bars represent mean ± SE (n = 3).*P<0.05, compared to HKM; γP<0.05, compared to HKM+Sc; #P<0.05, compared to HKM+MF; •P<0.05, compared to HKM+MF+Sc. HKM, control head kidney macrophage; HKM+STS, HKM treated with STS (positive control); HKM+Sc, HKM transfected with scrambled siRNA; HKM+MF, HKM infected with M. fortuitum; HKM+MF+Sc, HKM transfected with scrambled siRNA infected with M. fortuitum; HKM+MF+siRNA-CaM, HKM transfected with siRNA-CaM infected with M. fortuitum; HKM+MF+siRNA-CaMKIIg, HKM transfected with siRNA-CaMKIIg infected with M. fortuitum; HKM+MF+siRNA-NOS2, HKM transfected with siRNA-NOS2 infected with M. fortuitum; HKM+MF+BAPTA/AM, HKM+MF+CMZ, HKM+MF+CC, HKM+MF+Gö6976, HKM+MF+APO, HKM+MF+DPI, HKM+MF+KN-93, HKM+MF+KN-92, HKM+MF+GW5074, HKM+MF+PD98059, HKM+MF+U0126, HKM+MF+LNAME, HKM+MF+AMG, HKM+MF+Z-VAD-FMK, HKM+MF+Z-IETD-FMK, HKM+MF+Z-DEVD-FMK, HKM pre-treated with BAPTA/AM, CMZ, CC, Gö6976, APO, DPI, KN-93, KN-92, GW5074, PD98059, U0126, LNAME, AMG, Z-VAD-FMK, Z-IETD-FMK or Z-DEVD-FMK respectively and infected with M. fortuitum.