Figure 8.

Detection and identification of Bradyrhizobiaceae microbes in FFPE autopsy lung tissues by FISH using the Cy-3 labelled Bradyrhizobiaceae-specific P1-2 probe. The Cy-3 positive signals (red) were identified in areas with many chronic inflammatory cells, their nuclei stained (blue) by DAPI (A & C). Positive signals could be found in areas with fulminant necrosis and alveolar degradation. Few viable cells with their nuclei positively stained by DAPI were present in this area (B). Positive signals were also seen associated with degenerating inflammatory cells and pneumocytes in the intra-alveolar space (D).