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. 2015 Nov 1;8(11):13871–13885.

Figure 6.

Figure 6

Expression of apoptosis factors caspase 3, bcl-2, and cytochrome C. (A) In-cell immunoblotting detected the expression level of caspase 3, bcl-2, and cytochrome C in GSN-SMMC7721, shGSN-SMMC7721, NC1-SMMC7721 and NC2-SMMC7721. The experiments were carried out in triplicate. (B) Experiments were performed as in (A), the results showed the expression of caspase 3 was down-regulated 0.625±0.133 (P<0.05), and the expression of bcl-2 was up-regulated 4.138±0.857 (P<0.05) in GSN-SMMC7721 while comparing to NC1-SMMC7721. The expression of caspase 3 was up-regulated 1.908±0.257 (P<0.05), and the expression of bcl-2 was down-regulated 0.411±0.122 (P<0.05) in shGSN-SMMC7721 while comparing to NC2-SMMC7721. But the expression of cytochrome C was invariable while GSN was overexpressed/inhibited.