Figure 4.

Significant increase of NET formation in patients with GC. Myeloperoxidase-deoxyribonucleic acid complex (MPO-DNA), cell-free nucleosomes, and neutrophil elastase were measured with ELISA using plasma samples from healthy donors (CTR, n = 36), individuals with GC including patients in stage I (S-I, n = 7), stage II (S-II, n = 9), stage III (S-III, n = 25) and stage IV (S-IV, n = 7) cancer. Plasma CFDNA was quantified with fluorescent quantification. (A) MPO-DNA complex, (B) CFDNA, (C) nucleosomes, and (D) NE in patients with GC were increased with disease progression. Data are expressed as means ± standard deviation. *P < 0.001. GC, gastric cancer; OD, optical density; MPO, myeloperoxidase; CFDNA, cell-free DNA; NE, neutrophil elastase.