Figure 4.

Overexpression of PAPPA impaired the miR-141-induced inhibition of proliferation in VSMCs. A. Western blot analysis of PAPPA expression in VSMCs co-transfected with either miR-141 mimics or scramble and pCDNA-PAPPA or pCDNA empty vector; GAPDH was also detected as a loading control. B. Cell growth curves in the VSMCs transfected with different combinations. C. The protein expression of cyclin D1 was detected in the VSMCs transfected with different combinations using western blot. D. The mRNA expression of cyclin D1 was detected in the VSMCs transfected with different combinations using real-time PCR.