Figure 2.

Prediction of target gene for miR-96. A. Investigate the underlying mechanisms, we predicted the potential targeted genes of miR-96 by bioinformatics algorithms. Among all predicted genes, mTOR was found to be a target for miR-96. B. We found that miR-96 significantly reduced the luciferase activity of reporter for mTOR, while aso miR-96 markblly increased the luciferase activity of 3’UTR reporter for mTOR. C. mTOR mRNA and protein expression levels transfected with NC, miR-96, and ASO- miR-96 were analyzed by using western blotting. D. The relative luciferase active of the 3’UTR of target gene for miR-96 in different group. All experiments were performed in triplicate the significance in change in protein expression was analyzed by using statistical tests.