Figure 3.

Effects of PDLSCs on the osteoclastogenesis in RAW264.7 cells in the co-culture system. The formation of TRAP-positive, multinuclear cells (arrows) was increased in the presence of PDLSCs vs. control group. A. TRAP-positive cells were identified with microscope (original magnification 200×). B. The number of TRAP-positive multinucleated cells was counted in ten 200× fields. C, D. Expressions of TRAP, TRAF6 and CTSK in RAW264.7 cells co-cultured with PDLSCs analyzed by real time-PCR. C. Gene expression after 3 days of induction. D. Gene expression after 7 days of induction. E. Time-related protein expressions of TRAP and TRAF6 in RAW264.7 cells analyzed by Western blot. F. Quantitative analysis of the protein expression of TRACP. G. Quantitative analysis of the protein expression of TRAF6. *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group.