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Sites of NKX2-5-specific primers
| Primer | SNPs | Forward primer (5’-3’) | Reverse primer (5’-3’) | Product (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
| N1 | rs17052019 | TCTCCTGCCCCTTGTGCTCA | CGTAGGCCTCTGGCTTGAAGG | 332 | 61 |
| rs2277923 | |||||
| N2 | rs3729938 | TGGAGAAGACAGAGGCGGACAA | CGTAGGCGTTATAACCGTAGGGAT | 396 | 63 |
| rs3729753 | |||||
| rs3729754 | |||||
| N3 | rs703752 | ACAACAACTTCGTGAACTTCGG | ATCGTCATTTCTTACAGCAATAGGT | 542 | 58 |
| rs11552707 |
