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Primers used in real-time PCR
| Gene | Accesion NO. | Primer (5’-3’) |
|---|---|---|
| Smad4 | NM_005359 | F: CTCATGTGATCTATGCCCGTC |
| R: AGGTGATACAACTCGTTCGTAGT | ||
| NFκB p65 | NM_001145138 | F: ATGTGGAGATCATTGAGCAGC |
| R: CCTGGTCCTGTGTAGCCATT | ||
| Cyclin D3 | NM_001136017 | F: TACCCGCCATCCATGATCG |
| R: AGGCAGTCCACTTCAGTGC | ||
| GAPDH | NM_002046 | F: GAAGGTGAAGGTCGGAGTC |
| R: GAAGATGGTGATGGGATTTC |
F: forward; R: reverse.
