Table 2.
Synthetic system of PCR
| Components | Volume per reaction |
|---|---|
| SYBR Green Super mix | 5 μl |
| Forward primer (10 μM) | 0.3-0.55 μl (270-450 nM)2 |
| Reverse primer (10 μM) | 0.3-0.55 μl (270-450 nM) |
| cDNA template | 75-100 ng |
| Nuclease-free water | Up to 10 μl |
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