Table 1.
SNP | Primers | Primer sequence | Length of amplified fragments/bp | Restriction enzyme |
---|---|---|---|---|
C1236T | Forward | 5’-TTTCTCACTCGTCCTGGTA-3’ | 502 | EcoO109I |
Reverse | 5’-GTCATAGGCATTGGCTTC-3’ | |||
C3435T | Forward | 5’-TGTGCCTGGTCCTGAAGTT-3’ | 246 | Dpn |
Reverse | 5’-TAGGCAGTGACTCGATGAA-3’ |