Figure 1.

Lack of TRIM5α-Mediated Restriction in Human and Non-human Primate Myeloid DCs

(A–C) PBLs and DCs from (A) rhesus macaque, (B) human, and (C) African green monkey were transduced at MOI 5, ± nevirapine (5 μM), tenofovir disoproxil fumarate (TDF, 100 μM), heat-treated, or treated with a specific siRNA against human and RM TRIM5α (siHM3). Representative flow cytometry charts at 3 dpt are shown left, while graphs show the mean of three independent experiments with log₁₀ scale ± SD using different primate donors (RM, n = 2; human, n = 4; AGM, n = 2) and different vector batches.

(D) PBLs and DCs from cynomolgus macaque (n = 2), human (n = 3) and pigtailed macaque (n = 3) were transduced with increasing MOI ranging from 0.5 to 50. Graphs show GFP expression 3 dpt (3 independent experiments ± SD).

See also Figure S1.