Figure 1.

Detection of DHL by FISH. The G-banded karyotype was 49,XY,+8,t(8;14)(q24;q32),+12, t(14;18)(q32;q21),+r[11]/46,XY[18]. (A) FISH using the LSI MYC (8q24) dual color break apart rearrangement probe (Vysis) showed two intact copies of MYC and one rearranged (split) MYC signal. In metaphases the rearranged 5’MYC signal (red) was located on the derivative chromosome 8 [der(8)der(8;14)] and the 3’MYC signal (green) was located on a derivative chromosome 14 [the der(14)t(8;14)]. (B) FISH using the LSI IGH/BCL2 t(14;18) dual color dual fusion translocation probe (Vysis) identified two IGH/BCL2 fusion signals: one on a derivative chromosome 14 [der(14)t(14;18)] and one on the derivative chromosome 18 [der(18)t(14;18)]. Additional IGH signals (green) were seen on the der(8)t(8;14) and the der(14)t(8;14). A BCL2 signal (red) was present on the intact chromosome 18.

DHL, double hit lymphoma; FISH, fluorescence in situ hybridization.