Fig. 2.

The effect of manganese on DAergic neurons in immunofluorescence. 8 h, 1, 7, 14, and 28 days after manganese administration, immunofluorescence was conducted to measure TH-immunoreactivity and TH expression. A Location of substantia nigra pars compacta (SNpc). a Scale bar indicates 1,000 μm. b Scale bar indicates 500 μm. c Scale bar indicates 200 μm. B Quantitative analysis of the effect of manganese on DAergic neurons in the ratio of total neurons. *p < 0.05, **p < 0.01 compared with control groups. C The effect of manganese on TH-immunoreactivity. Scale bar indicates 200 μm