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. 2016 Jan;186(1):134–144. doi: 10.1016/j.ajpath.2015.09.013

Figure 3.

Figure 3

Proinflammatory cytokine mRNA expression increases after CRHR2 inhibition during recovery from DSS colitis. mRNA levels are elevated for TNF-α (A), IL-6 (C), CXCL1 (E), and the signal-transducing subunit of the IL-6 receptor (F) in mice injected intracolonically with Ast2B compared with vehicle-treated controls. Protein expression levels (in pg/mL) of TNF-α (B) and IL-6 (D) are also increased. G: CRHR2 mRNA expression is unchanged as a result of Ast2B injections, whereas transcripts for Ucn2 (H) are increased compared with controls. Data are expressed as means ± SEM. n = 6 to 8 mice per group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared with vehicle-treated controls. Ast2B, astressin 2B; CRHR2, corticotropin-releasing hormone receptor 2; DSS, dextran sodium sulfate; ST, signal transducer; TNF, tumor necrosis factor; Ucn2, urocortin 2.