Stimulation of CRHR2 enhances colonic epithelial cell viability. A: CRHR2-overexpressing NCM460 cells are protected against TNF-α–induced decreases in cell viability. B: Quantification of TUNEL. C: Representative images from TUNEL sections. Data are expressed as means ± SEM. n = 3 wells per group (A); n = 8 mice per group (B). ∗P < 0.05. Scale bar = 100 μm. Ast2B, astressin 2B; CRHR2, corticotropin-releasing hormone receptor 2; EV, empty vector; TNF, tumor necrosis factor; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.