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. 2016 Jan;186(1):134–144. doi: 10.1016/j.ajpath.2015.09.013

Figure 5.

Figure 5

Stimulation of CRHR2 promotes colonic epithelial cell proliferation. A and B: Human CRHR2-transfected NCM460 cells (CRHR2) and EV transfected cells were stimulated with varying concentrations of Ucn2 (A) or CRH (B) with or without pretreatment of 1 μmol/L Ast2B, and proliferation was assessed with the use of the slope (1/h) of data collected with the xCELLigence software. C: Representative images from Ki-67 immunoreactive sections. D: Quantification of Ki-67 immunohistochemistry with the use of pixel-based densitometry. Data are expressed as means ± SEM. n = 3 wells per group (A); n = 8 mice per group (D). ∗P < 0.05 compared with vehicle-treated controls; ∗∗∗P < 0.001 versus EV controls; †††P < 0.001 versus Ucn2 or CRH treatment of CRHR2 cells alone. Scale bar = 100 μm. Ast2B, astressin 2B; CHR, corticotropin-releasing hormone; CRHR2, corticotropin-releasing hormone receptor 2; EV, empty vector; Ucn2, urocortin 2.