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. 2016 Jan;186(1):134–144. doi: 10.1016/j.ajpath.2015.09.013

Figure 7.

Figure 7

Down-regulation of CRHR2 or STAT3 by siRNA inhibits colonic epithelial cell migration and proliferation. Cell migration was assayed by the slope (1/h) of data collected with xCELLigence in CRHR2-overexpressing NCM460 cells transfected with siRNA against CRHR2 (A), STAT3 (B), or siCTL after stimulation with 0.1 μmol/L Ucn2 and IL-6. C and D: Cell proliferation was measured with the slope (1/h) of data collected with xCELLigence in CRHR2 (C), STAT3 (D), or siCTL siRNA transfected CRHR2-overexpressing NCM460 cells after stimulation with 0.1 μmol/L Ucn2 and IL-6. Data are expressed as means ± SEM. n = 2 wells per group. ∗∗P < 0.01, ∗∗∗P < 0.001. CRHR2, corticotropin-releasing hormone receptor 2; siCTL, control siRNA; STAT3, signal transducer and activator of transcription 3; Ucn2, urocortin 2.