TABLE 4.
Osteoarthritis Studies: Cell Number and Type, Marrow Harvest and Concentration, Stratification of Defecta
Publication | Cell Number or Type of Cells Injected | Marrow Harvesting and Concentration | Stratification of Focal Cartilage Defect Severity |
---|---|---|---|
Centeno et al2 | Approximately 10-15 mL of bone marrow aspirate was withdrawn from 6-8 sites. Isolation produced 1-3 mL of BMC injectate, which was then transported via sterile means back to the operating room. Injected with PRP and also lipoaspirate in a second group. | The aspirate was processed by hand in a sterile ISO-7 class clean room and in ISO-5 class laminar flow cabinets to isolate the buffy coat through centrifugation. | KL 2 patients were significantly more likely (2.2 times) to report ≥50% improvement on the reported outcome scale in comparison with the reference group (KL 3-4 grade). |
Kim et al16 | Calculated estimation based off of 7 mL of bone marrow–derived mesenchymal stem cells and 10 mL of adipose tissues: 2.4 × 105 adult stem cells and 1.8 × 109 mononuclear cells. | Autologous bone marrow of 120 mL is aspirated from ASIS or PSIS of the pelvis by using SmartPReP2 Bone Marrow Procedure Pack BMAC2 kits. | KL 1, 2, 3, 4 (12, 24, 33, 6 patients, respectively) (better results KL 1-3; poor results, KL 4). |
Hauser and Orlofsky et al12 | Whole bone marrow/not fractioned/marrow adipocytes | Not concentrated | Not reported |
aASIS, anterior superior iliac spine; BMC, bone marrow concentrate; BMAC, bone marrow aspirate concentrate; ISO, International Organization for Standardization; KL, Kellgren-Lawrence; PRP, platelet-rich plasma; PSIS, posterior superior iliac spine.