Figure. 3.

Diabetic conditions suppress phospho-AMPKα, as determined by Western blotting. The major band for phospho-AMPKα protein at 62 kDa decreased in high glucose conditions, B30 and A30, (A) at 24 hours and 48 hours in rat GEpCs and (B) in A30 at 48 hours in mouse podocytes. Data on the densitometric analysis of the phospho-AMPKα/total AMPK or β-tubulin ratio are expressed as mean±SD (n=3). Control (100%); the value of the B5 condition. ^⁎P<0.05 and ^†P<0.01 versus control. AMPK, adenosine monophosphate-activated protein kinase; GEpCs, glomerular epithelial cells; SD, standard deviation.