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. 2015 Nov 3;291(3):1029–1052. doi: 10.1074/jbc.M115.689653

FIGURE 6.

FIGURE 6.

Novel thiosemicarbazones, Dp44mT and DpC, decrease EGFR expression at the cell membrane. PANC-1 (A) and HT-29 (B) cells were incubated for 24 h at 37 °C with control media (Ctrl) or media containing the following: Dp2mT (10 μm), DFO (250 μm), Dp44mT (10 μm), or DpC (10 μm), and levels of NDRG1 and EGFR were examined by Western blot. β-Actin was used as a loading control. Western blots are typical of three independent experiments, with densitometric analysis representing mean ± S.D. (three experiments). Relative to untreated control cells: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Relative to DFO-treated cells: #, p < 0.05. EGFR localization was examined via immunofluorescence in PANC-1 (C) and HT-29 (D) cells that were incubated for 24 h at 37 °C with either control media or media containing Dp2mT (10 μm), DFO (250 μm), Dp44mT (10 μm), or DpC (10 μm). The scale bar in the bottom right corner of the first image represents 50 μm and is the same across all images. Results are typical of three independent experiments.