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. 2015 Nov 3;291(3):1029–1052. doi: 10.1074/jbc.M115.689653

FIGURE 9.

FIGURE 9.

Effect of DpC on inhibiting EGFR expression and activation occurs independently of NDRG1 in the presence of the EGF ligand. PANC-1 (A) and HT-29 (B) cells were transiently transfected with nonspecific control siRNA (siControl) or NDRG1 siRNA (siNDRG1) for 72 h at 37 °C, followed by treatment with Dp2mT (10 μm), DFO (250 μm), Dp44mT (10 μm), or DpC (10 μm) for 24 h at 37 °C with EGF (10 ng/ml) added for the last 10 min of the 24-h incubation. NDRG1, EGFR, and pEGFR(Tyr-1068) levels were then examined by Western blot, with β-actin used as a loading control. Western blots are typical of three independent experiments, with densitometric analysis representing mean ± S.D. (three experiments). Relative to untreated control cells: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Relative to the correspondingly treated siControl cells: #, p < 0.05.