FIGURE 6.
Molecular interactions between MFAP4 and fibrillins. A, BIAcore analysis of binding between immobilized MFAP4 and soluble fibrillin-1 fragments flown over at 100 nm in the presence of 5 mm CaCl2. One representative experiment is shown. B, BIAcore analysis of binding between immobilized MFAP4 and soluble fibrillin-2 fragments flown over at 100 nm in the presence of 5 mm CaCl2. One representative experiment is shown. C, structure of full-length fibrillin-1 and fibrillin-2 with identified MFAP4 binding sites (red rectangles). D, confocal immunofluorescence microscopy shows co-localization of MFAP4 (red) and fibrillin-1 (green) in the skin of WT and GT-8 mice (GT-8/GT-8) at postnatal days P1, P7, and P14. Representative pictures are shown. Original magnification, 126×. Scale bar, 20 μm.