FIGURE 8.

Molecular interactions between MFAP4 and desmosine. A, MFAP4 binds immobilized ovalbumin-desmosine conjugate in a calcium-dependent manner in solid phase binding assays. The background binding to ovalbumin was subtracted from the results. B, BIAcore analysis of binding between immobilized MFAP4 and soluble ovalbumin-desmosine conjugate and ovalbumin control. One representative experiment is shown. C, mutations of the S1 binding site modify MFAP4-desmosine binding capacity. The data are means ± S.E. of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001, calculated by Student's t test.