FIGURE 2.

QS-21 induces caspase-1/11-dependent IL-1β secretion. Murine BMDCs (A) or macrophages (B) from WT or caspase-1/11-deficient mice were stimulated for 6 h with alum (100 μg/ml) or QS-21 (10 or 2 μg/ml) with or without MPLA (5 μg/ml). IL-6 and IL-1β were measured in culture supernatants by ELISA. The data are triplicates ± S.E. and are representative of two or more independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 indicate a significant difference detected between WT and caspase-1/11 cells determined by Student's t test.