FIGURE 6.

Other QS-21-containing saponins activate IL-1β secretion through NLRP3. Murine macrophages from WT or NLRP3-deficient mice were stimulated for 6 h with the indicated stimulants (5 μg/ml for all except 2 μg/ml for QS-21) in the presence or absence of MPLA (5 μg/ml) (A and B). IL-1β and IL-6 were measured in culture supernatants by ELISA. The data are triplicates ± S.E. and are representative of two or more independent experiments. *, p < 0.05 indicates a significant difference determined by Student's t test.