TABLE 1.

Kinetic parameters for the hydrolysis of various substrates by PbGH5A

ND, not determinable.

Substrate	kcat	Km	kcat/Km	Assay
	min−1	mm	m−1s−1
bMLG	3.49 ± 0.05 × 104	0.122 ± 0.007a	4.8 × 103a	BCA
tXyG	6.8 ± 0.4 × 103	1.1 ± 0.2a	103a	BCA
kGM	3.2 ± 0.2 × 103	0.90 ± 0.17a	59a	BCA
cGM	ND	ND	ND	BCA
CMC	1.83 ± 0.14 × 103	2.3 ± 0.4a	13a	BCA
HEC	2.0 ± 0.2 × 103	0.62 ± 0.13a	54a	BCA
PASC	2.0 ± 0.2 × 103	10.8 ± 1.3a	3.1a	BCA
wAX	ND	ND	0.23 ± 0.04	BCA
bX	ND	ND	0.23 ± 0.03	BCA
G-PNP	ND	ND	ND	PNP
GG-PNP	ND	ND	4.4 ± 0.2	PNP
GG-CNP	460 ± 30	11.4 ± 1.3	670	CNP
GGG-PNP	360 ± 25	9.2 ± 1.2	650	PNP
GGG-CNP	9.1 ± 0.3 × 103	1.5 + 0.1	1.01 × 105	CNP
GGGG-CNP	8.1 ± 0.4 × 103	0.7 ± 0.1	1.94 × 105	CNP
XXXG-CNP	9.7 ± 0.8 × 103	0.064 ± 0.013	2.53 × 106	CNP
G3GGG3GGG→G3GGG+GGG	1.97 ± 0.13 × 104	0.570 ± 0.040	5.8 × 105	HPAEC-PAD
G3GGG	ND	ND	ND	HPAEC-PAD
GG3GG→GG+GG	ND	ND	1.46 ± 0.2 × 103	HPAEC-PAD
GGG3G→GGG+G	ND	ND	3.13 ± 0.4 × 103	HPAEC-PAD
GGG3G→GG+G3G	ND	ND	600 ± 200	HPAEC-PAD
GGGG→GG+GG	106 ± 4	0.77 ± 0.05	2.3 × 103	HPAEC-PAD
GGGG→GGG+G	ND	ND	300 ± 50	HPAEC-PAD
GGGGG→GGG+GG	1.21 ± 0.02 × 104	0.753 ± 0.026	2.68 × 105	HPAEC-PAD
GGGGGG→GGGG+GG	9.3 ± 1.5 × 103	0.79 ± 0.22	1.96 × 105	HPAEC-PAD
GGGGGG→GGG+GGG	6.3 ± 1.1 × 103	0.84 ± 0.26	1.25 × 105	HPAEC-PAD
XXXGXXXG→2XXXXG	422 ± 10	0.037 ± 0.009	1.9 × 105	HPAEC-PAD
MMMMMM	ND	ND	ND	HPAEC-PAD

^a Concentrations are expressed as g/liter. Apparent K_m values are reported.