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. 2015 Nov 18;291(3):1221–1234. doi: 10.1074/jbc.M115.677682

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — LFG subcellular localization. A, representative confocal images of cortical neurons. Cortical neurons were fixed, permeabilized, and immunostained with anti-LFG (green), Mitotracker (mitochondria marker), Hoechst (nucleus marker), anti-calnexin (ER marker), anti-Rab5 (early endosome marker), and anti-GM130 (Golgi marker) (red). The third column shows a merge of the green and red channels. Scale bar, 10 μm. B, Manders' co-localization coefficients for LFG are shown as percentage of LFG co-localization with each marker (one-way ANOVA test). ***, p ≤ 0.001; **, p ≤ 0.005; *, p ≤ 0.05). The values of all Manders' co-localization coefficients and Pearson correlation coefficients for each condition are detailed under “Results.”