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. 2015 Nov 12;291(3):1277–1288. doi: 10.1074/jbc.M115.684100

FIGURE 1.

FIGURE 1.

The steps in construction of the S. paradoxus strains. A, the HO gene was disrupted by replacement with the bacterial KANMX6 gene. B, the ADE1 gene in S. paradoxus was disrupted by the URA3 gene from S. cerevisiae (URA3Sc). C, the ade1–14 and ura3 mutations were engineered by replacing URA3Sc (previously inserted to disrupt ADE1) with ade1–14Sc. D, the SUP35 gene in a S. paradoxus chromosome was replaced by natNT2 (conveying resistance to nourseothricin). E, an S. paradoxus strain having both ade1–14 and sup35Δ::natNT2 in its genotype was obtained by crossing two S. paradoxus yeast strains (one containing ade1–14, and another containing sup35Δ::natNT2 and a wild-type copy of SUP35 on a plasmid) of the opposite mating type. This was followed by sporulation and dissection.