FIGURE 2.

Cross-species prion transfection. A, summary of the transfection procedure. B, cellular extract was transfected from either a strong or weak [PSI⁺] S. cerevisiae strain into a [psi⁻] S. paradoxus strain, expressing the Sup35 protein from S. cerevisiae. Representative [PSI⁺] S. paradoxus transfectants obtained from either the strong (left) or weak (right) [PSI⁺] donor strains are shown. Yeast cells were grown on YPD (for the color assay) and on -Ade medium (for the suppression assay) at 30 °C for 8 days.