FIGURE 5.

Comparison of reverse cross-species prion transmission in S. cerevisiae and S. paradoxus. The frequencies of [PSI⁺] transmission from the divergent or chimeric prion domains (as indicated) to the S. cerevisiae protein are shown. Notably, the reverse shuffle constructs containing the S. paradoxus PrD or its module I restored the original strong [PSI⁺] variant. In contrast, reverse shuffle from constructs containing module II of S. bayanus produced weak [PSI⁺] variants. I, II, and III refer to the exchangeable modules of the PrD.