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. 2015 Dec 2;291(3):1307–1319. doi: 10.1074/jbc.M115.691766

FIGURE 3.

FIGURE 3.

PEPCK-C counteracts loss of cellular function and integrity with age and senescence. A, self-fertile reproductive span of control and PEPCK-C OE in BDWM. *, p < 0.05; t test; n = 78–80. B, ROS in day 1 WT (Ctrl) and PEPCK-C KO, rol-6 (Ctrl), and rol-6 with PEPCK-C OE in whole body (n = 3). p < 0.05, t test. C, survival of rol-6 (Ctrl) and rol-6 with PEPCK-C OE in whole body. D, survival of WT and PEPCK-C KO. E-G, deposit of age-pigments (E) and β-galactosidase (F and G) of control and PEPCK-C OE in BDWM (n = 51–97 (E) or 12 (G)). *, p < 0.05; two-way analysis of variance (E) or t test (G). F, β-galactosidase deposit with age in WT. G, quantification of β-galactosidase deposit in day 15 WT and PEPCK-C OE in BDWM. H, immunoblotting against CKI-1 (n = 3). *, p < 0.05; t test. Upper, Sample images. &, nonspecific bands. Lower, immunoreactivity of CKI-1 over β-tubulin in arbitrary unit.