FIGURE 6.

Rapamycin reverses HFD-induced Miox expression and associated changes in the renal tubules. A significant increase in body weight, blood glucose, and serum insulin levels was observed following HFD administration, and it was reduced following rapamycin treatment (A, C, and D). HFD-induced pathologic changes, i.e. loss of proximal tubular brush border and extrusion of the nuclei (arrowheads) were reversed by rapamycin treatment (E–G). Also, the expression of Miox (mRNA and protein), Bax, and pH2AX was restored to basal levels following rapamycin treatment (B and H–P). *, p < 0.01 versus control, n = 4.