FIGURE 9.

srebp1 and miox siRNA reduced palmitate/BSA-induced perturbations in cellular redox in renal tubular cells. A significant increase in MFI, as assessed by flow cytometric analyses, following palmitate/BSA treatment was observed (B versus A and E). srebp1 siRNA treatment reduced the MFI (C and E). Similarly, a reduction in MFI was observed following miox siRNA treatment (D and E). Likewise fluorescent microscopy of HK-2 cells revealed a notable increase in intensity of staining following palmitate/BSA treatment (G versus F). Both the srebp1 siRNA and miox siRNA treatments remarkably reduced intensity of fluorescence, suggesting that palmitate/BSA-induced ROS perturbations are specific to miox and are mediated via Srebp1 (H and I). *, p < 0.01 versus control, n = 4.