FIGURE 4.
P-selectin-dependent neutrophil rolling and migration in SelpKI/KI and SelpKI/− mice. A, mean leukocyte rolling velocities in trauma-stimulated venules (n = 150–200 leukocytes from 15–20 venules/group) or in TNF-α-stimulated venules of mice injected with blocking anti-E-selectin mAb (anti-E-sel) (n = 320–330 leukocytes from 32–33 venules/group). B, WT, SelpKI/KI,or SelpKI/− mice were injected intraperitoneally with thioglycollate. Some mice were injected intravenously with blocking anti-P-selectin mAb (anti-P-sel) at the time of thioglycollate challenge. After 4 h, peritoneal cells were lavaged, and neutrophils were quantified by flow cytometry (n = 7–10 mice/group). *, p < 0.05.