FIGURE 5.

Single-stranded DNA translocation activity of DndEih. Translocation activity was investigated by measuring the ability of DndEih to displace streptavidin from a biotinylated probe at the 5′ or 3′ end of ssDNA. The black circle indicates the biotin-labeled DNA end, with the larger circle representing the streptavidin. Lane 1, biotinylated DNA; lane 2, biotinylated DNA bound to streptavidin; lane 3, streptavidin-bound DNA incubated with DndEih for 30 min in the absence of ATP; lane 4, streptavidin-bound DNA incubated with DndEih for 30 min in the presence of ATP.