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. 2015 Dec 2;291(3):1492–1500. doi: 10.1074/jbc.M115.694018

FIGURE 7.

FIGURE 7.

Analysis of the PT modification in R. anatipestifer HXb2 harboring a pReS0-based plasmid (pJ1–5), which has the PT-modifying genes from R. anatipestifer ATCC 11845. A, schematic diagram of the PT-modifying genes in pReS0-based plasmids (Table 1). B, electrophoresis pattern of the iodine-cleaved genomic DNA from R. anatipestifer HXb2 (pJ1) (lanes 4 and 5), HXb2 (pJ2) (lanes 6 and 7), HXb2 (pJ3) (lanes 8 and 9), HXb2 (pJ4) (lanes 10 and 11), HXb2 (pJ5) (lanes 12 and 13), and HXb2 (pReS0 empty vector) (lanes 2 and 3). M, DNA ladder. C, LC-MS analysis of the PT-linked dinucleotides from R. anatipestifer HXb2 harboring a pReS0-based plasmid. Electrospray ionization: m/z [M + H]+ modes for LC-MS: d(GpsA), 597.1388; d(GpsT), 588.1272.